Introducing the CHO-MK Platform
CHITOSE’s proprietary CHO-MK* is a host cell line developed from a primary culture of healthy Chinese hamster ovary tissue and is fully banked for biopharmaceutical production.
In performance testing, CHO-MK has demonstrated remarkably higher productivity compared to conventional CHO cell lines.
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Naturally established without viral vectors or genetic engineering.
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Yielding high titers in serum-free suspension culture.
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A stable chromosomal foundation for consistent, long-term manufacturing.
*CHO-MK cells were developed under the support of AMED (Grant Number JP18ae101054)
CHO-MK Characteristics & Performance
The CHO-MK platform redefines bioprocess efficiency, consistently achieving antibody titers exceeding 10 g/L* within a condensed 5 – 7 day production cycle. This high productivity maximizes facility throughput and significantly lowers operational costs.
Our lead clones exhibit excellent phenotypic stability and have been proven for robust performance at the 2,000 L manufacturing scale, ensuring a seamless transition from development to commercial production.
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High Titers: Deliver consistently high yields of 10 – 13 g/L.
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Industry Speed: Rapid 5 – 7 day production cycles.
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Scalable Growth: Optimized for high-density suspension.
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Proven Stability: Scalable manufacturing.
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Fully banked industrial cell lines.
10 – 13 g/L
antibody yields*
5 – 7 Days
Production Cycle
>2,000 L
bioreactor scale
*Enhancing monoclonal antibody production efficiency using CHO-MK cells and specific media in a conventional fed-batch culture, Hisashi Saeki, et al, Cytotechnology (2025) Volume 77 (1)
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CS CHO Expression Vector
Developed through over a decade of protein expression research, the CHITOSE Super CHO (CS CHO) Expression Vector is our proprietary system engineered for high-performance biopharmaceutical production.
By integrating the optimized signal sequences and nucleotide designs, this vector ensures robust protein expression specifically fine-tuned for the CHO-MK host platform.
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CHITOSE's original expression vector system
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Contains optimized signal sequences to ensure high-level protein expression
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Features expertly designed nucleotide sequences for maximizing expression in CHO-MK cells
Cell Line Development Service
From DNA to pCB with high‑titer, stable CHO-MK cell lines
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CHO-MK Cell Line and CS CHO expression vector system
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FCM-based enrichment of highly producing stable pools
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High-fidelity cloning by single-cell sorting
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Clonality assurance by high-resolution imaging
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Early process development using bioreactor
Built on over a decade of expertise, CHITOSE has established a high-performance protein expression platform integrating our productive CHO-MK host cell line and proprietary CS CHO expression vector system.
We deliver robust, flexible solutions that shorten production timelines and reduce costs for biologics development, providing a reliable expression system for your protein through high-performance Research Cell Banks (RCBs) and Pre-Master Cell Banks (PCBs).
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Vector Construction ~ Stable Pool
Cloning ~ RCB
Passage Stability ~ PCB
Process Dev. CharACTERIZATION
- DNA & Vector Synthesis
- Vector Construction
- Stable Pool Enrichment
- Single Cell Cloning
- Clonality Assurance
- High Expression Clone Selection
- Passage Stability Testing
- PCB Candidate Selection
- Early Process Development
- Technology Transfer
Cell Line Development Process
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Speedy Cell Line Development Timeline
Vector Construction
Clone Production
PCB Production
PCB Characteristic Analysis
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Months
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REDUCED COST OF GOODS • HIGH-PRODUCTIVITY • SCALABLE AND EFFICIENT • SHORT PRODUCTION TIME • STREAMLINED PROCESS • CONSISTENT APPROACH •
Business Process
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Scope &
Requirement
Definition
Timeline &
Quotation
Proposal
Service
Implementation &
Support
Initial Inquiry
NDA Signing
Contract Execution
How We Support You
Comprehensive Partner Network
Integrated connectivity with media manufacturers, CROs, and equipment suppliers through a robust, expert-led network.
One-Stop Solution
A unified hub delivering end-to-end support for pharmaceutical and biotech companies, simplifying complex bioprocesses.
One-Stop Solution
A unified hub delivering end-to-end support for pharmaceutical and biotech companies, simplifying complex bioprocesses.
Single Point of Contact
Acting as your lead coordinator to ensure seamless transitions from initial development to commercial-scale manufacturing.
Single Point of Contact
Acting as your lead coordinator to ensure seamless transitions from initial development to commercial-scale manufacturing.
Technical Documentation
Providing comprehensive documentation upon request.
Technical Document
Providing comprehensive documentation upon request