Introducing the CHO-MK Platform
CHITOSE’s proprietary CHO-MK* is a host cell line developed from a primary culture of healthy Chinese hamster ovary tissue and is fully banked for biopharmaceutical production.
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In performance testing, CHO-MK has demonstrated remarkably higher productivity compared to conventional CHO cell lines.
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Naturally established without viral vectors or genetic engineering.
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Yielding high titers in serum-free suspension culture.
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A stable chromosomal foundation for consistent, long-term manufacturing.
*CHO-MK cells were developed under the support of AMED (Grant Number JP18ae101054)
CHO-MK Characteristics & Performance
The CHO-MK platform redefines bioprocess efficiency, consistently achieving antibody titers exceeding 10 g/L* within a condensed 5 – 7 day production cycle. This high productivity maximizes facility throughput and significantly lowers operational costs.
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Our lead clones exhibit excellent phenotypic stability and have been proven for robust performance at the 2,000 L manufacturing scale, ensuring a seamless transition from development to commercial production.
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High Titers: Deliver consistently high yields of 10 – 13 g/L.
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Industry Speed: Rapid 5 – 7 day production cycles.
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Scalable Growth: Optimized for high-density suspension.
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Proven Stability: Scalable manufacturing.
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Fully banked industrial cell lines.
10 – 13 g/LÂ
antibody yields*
5 – 7 Days
Production Cycle
>2,000 L
bioreactor scale
*Enhancing monoclonal antibody production efficiency using CHO-MK cells and specific media in a conventional fed-batch culture, Hisashi Saeki, et al, Cytotechnology (2025) Volume 77 (1)
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CS CHO Expression Vector
Developed through over a decade of protein expression research, the CHITOSE Super CHO (CS CHO) Expression Vector is our proprietary system engineered for high-performance biopharmaceutical production.
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By integrating the optimized signal sequences and nucleotide designs, this vector ensures robust protein expression specifically fine-tuned for the CHO-MK host platform.
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CHITOSE's original expression vector system
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Contains optimized signal sequences to ensure high-level protein expression
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Features expertly designed nucleotide sequences for maximizing expression in CHO-MK cells
Cell Line Development Service
From DNA to pCB with high‑titer, stable CHO-MK cell lines
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CHO-MK Cell Line and CS CHO expression vector system
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FCM-based enrichment of highly producing stable pools
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High-fidelity cloning by single-cell sorting
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Clonality assurance by high-resolution imaging
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Early process development using bioreactor
Built on over a decade of expertise, CHITOSE has established a high-performance protein expression platform integrating our productive CHO-MK host cell line and proprietary CS CHO expression vector system.
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We deliver robust, flexible solutions that shorten production timelines and reduce costs for biologics development, providing a reliable expression system for your protein through high-performance Research Cell Banks (RCBs) and Pre-Master Cell Banks (PCBs).
Step 1
Step 2
Step 3
Step 4
Vector Construction ~ Stable Pool
Cloning ~ RCB
Passage Stability ~ PCB
Process Dev. CharACTERIZATION
- DNA & Vector Synthesis
- Vector Construction
- Stable Pool Enrichment
- Single Cell Cloning
- Clonality Assurance
- High Expression Clone Selection
- Passage Stability Testing
- PCB Candidate Selection​
- Early Process Development
- Technology Transfer
Cell Line Development Process
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1
Speedy Cell Line Development Timeline
Vector Construction
Clone Production
PCB Production
PCB Characteristic Analysis
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4
Months
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REDUCED COST OF GOODS • HIGH-PRODUCTIVITY • SCALABLE AND EFFICIENT • SHORT PRODUCTION TIME • STREAMLINED PROCESS • CONSISTENT APPROACH •
Business Process
Step 1
Step 2
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Step 4
Step 5
Initial Inquiry
NDA Signing
Technical Consultation, Commercial Terms
& Quotation
Contract Execution
Service
Implementation &
Support
How We Support You
Comprehensive Partner Network
Integrated connectivity with media manufacturers, CROs, and equipment suppliers through a robust, expert-led network.
One-Stop Solution
A unified hub delivering end-to-end support for pharmaceutical and biotech companies, simplifying complex bioprocesses.
One-Stop Solution
A unified hub delivering end-to-end support for pharmaceutical and biotech companies, simplifying complex bioprocesses.
Single Point of Contact
Acting as your lead coordinator to ensure seamless transitions from initial development to commercial-scale manufacturing.
Single Point of Contact
Acting as your lead coordinator to ensure seamless transitions from initial development to commercial-scale manufacturing.
Technical Documentation
Providing comprehensive documentation upon request.
Technical Document
Providing comprehensive documentation upon request